uid=LUQ,o=LTER,dc=ecoinformatics,dc=org all public read LUQMetadata160 Sharon Cantrell Universidad del Turabo

Universidad del Turabo Science and Technology P. O. Box 3030 Gurabo PR 778 United States

(787) 743-7979 x4170 scantrell@lternet.edu Metadata Provider Metadata Provider Luquillo LTER

Anexo Facundo Bueso Build Rio Piedras PR United States

(787) 764-0000 emelendez@lternet.edu Eda Melendez-Colom University of Puerto Rico

University of Puerto Rico at Rio Piedras, Ponce de Leon Ave.,Anexo Facundo Bueso Office 207 San Juan PR 931 United States

(787) 764-0000 x4943 (787) 772-1481 emelendez@lternet.edu data manager Francisco Rivera Universidad del Turabo

Science and Technology Universidad del Turabo P. O. Box 3030 Gurabo PR 778 United States

(787) 743-7979 x4170 (787) 743-7979 x4041 frivera@lternet.edu associate researcher Jean Lodge NRS, USDA Forest Service

USDA - Forest Services Center for Forest Mycology Research, Northern Research Station PO Box 1377 Luquillo PR 773 United States

(787) 889-7445 (787) 889-7477 dlodge@lternet.edu associate researcher Marirosa Molina U.S. Environmental Protection Agency

Ecosystems Research Division National Exposure Research Laboratory Office of Research and Development U.S. Environmental Protection Agency 960 College Station Road Athens GA 30605 United States

(706) 355-8113 molina.marirosa@epa.gov associate researcher english

The canopy trimming experiment at El Verde simulates some aspects (canopy openness and biomass redistribution) of hurricane disturbances. Soil samples and leaf litter were gathered from three replicate blocks, each with four treatment plots in Tabonuco Forest at in El Verde. Treatments (canopy trimming and debris addition) were applied in a 2 x 2 factorial design. Samples were obtained both before and after the canopy were trimmed and debris was applied in the appropriate treatments. Samples were collected every four months before and after treatments were applied. Molecular approaches such as EL-FAME are useful indicators of microbial community shifts in response to environmental change. In this experiment we analyzed microbial community composition and abundance in soil and leaf litter samples as reflected by EL-FAME profiles. All soil samples were cleaned by removing rocks and roots, and leaf litter samples were ground.Fatty acid nomenclature: Fatty acids are named according to the conversion X:YωZ, where X represents the number of carbon atoms in the chain, followed by Y after the colon which represents the degree of the unsaturation. The symbol ω and Z represent the number of double bonds nearest to the carboxyl end. The prefixes a, i, cy and d refer to anteiso, iso, cyclopropyl branching and dicarboxylic fatty acid respectively; br indicates that the type of branching is unknown, while a number followed by Me indicates position of methyl group. Prefixes a and b indicate that the OH groups of an OH fatty acid are located at positions 2 and 3 respectively. Numbers preceded by w indicate the position of OH groups from the aliphatic end of the fatty acids (Kaur et al 2005).Community analysis based on fatty acids: Fatty acid biomarkers could represent a group of particular microorganisms present in soil and leaf litter. Fatty acids used in literature as biomarkers are: Branched chain fatty acids (br 17:0, br 18:0, i17:0, a17:0, i16:0, i16:1, 10Me16:0, 10Me17:0), iso and anteiso isomers of 15:0 for gram positive bacteria; Cyclopropane fatty acids (cy17:0, cy19:0, 16:1w9, 16:1w7c, 16:1w5, 18:1w7,19:1) for gram-negative bacteria; 18:2w6 for fungi; 10Me16:0, 10Me17 : 0 and 10Me18 : 0 for Actinomycetes; cy17:0 and 10Me16:0 for Sulphate reducing bacteria; 16:1w8, 18:1w8 for Methanogens (Modified from Zelles, 1999; Kaur et al 2005).

microbial communities ester-linked fatty acid methyl ester (EL-FAME) analysis LUQ Luquillo Data Sets Vocabulary V.2 (Researcher's Keywords) disturbance populations LTER_core_area Litterfall and coarse woody debris decompostion LUQ LTER 2006 Classification chemistry fatty acids soil litterfall On Table 4 Vocab

Copyright 2001 Long Term Ecological Research Network - See our standard http://luq.lternet.edu/datamng/usersagreement User Agreement and Disclaimer Page This material is based upon work supported by the National Science Foundation under Cooperative Agreements DEB-0620910 DEB-0218039 DEB-0080538 DEB-9705814 DEB-9411973 BSR-8811902 Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact LUQ webmaster with questions, comments, or for technical assistance regarding this web site.

http://hellokitty.ites.upr.edu/node/6796 2002-11-02 2006-03-31 Francisco Rivera Universidad del Turabo

Science and Technology Universidad del Turabo P. O. Box 3030 Gurabo PR 778 United States

(787) 743-7979 x4170 (787) 743-7979 x4041 frivera@lternet.edu Sharon Cantrell Universidad del Turabo

Universidad del Turabo Science and Technology P. O. Box 3030 Gurabo PR 778 United States

(787) 743-7979 x4170 scantrell@lternet.edu Publisher Publisher Luquillo LTER

Anexo Facundo Bueso Build Rio Piedras PR United States

(787) 764-0000 emelendez@lternet.edu LUQ

A PVC core (5cm diameter) was used to collect 0-10cm soil samples from five sub plots in each treatment plot. The five sub plot samples were clean (rocks, wood and invertebrates debris and roots) and pooled. Approximately 20 g of leaf litter samples were gathered close to where the soil cores were taken, then pooled and ground. All samples were kept at -4 °C after being pre-processed then moved to a -70 °C for longer storage.Lipids were extracted directly from soil and leaf litter using the EL-FAME method described by Schutter and Dick (2000). The lipid extraction procedure used a mild alkaline methylation, which in theory, remove the cellular ester linked fatty acid and not free fatty acids. After extracting the fatty acids by this method, they were cleaned with an amilopropyl (NH2) column to remove any humic substances. Fatty acid samples were kept frozen at -20 °C in darkness until their analysis in gas chromatograph.

CTE-SOIL_EL-FAME.txt Soil CTE-EL-FAME CTE-SOIL_EL-FAME.txt \n column , http://hellokitty.ites.upr.edu/sites/default/files/[field_dataset_datafile_ref-path]/data/CTE-SOIL_EL-FAME.txt 2002-11-01 2006-06-17 CTE-LEAF_LITTER_EL-FAME.txt Leaf Litter CTE-EL-FAME CTE-LEAF_LITTER_EL-FAME.txt \n column , http://hellokitty.ites.upr.edu/sites/default/files/[field_dataset_datafile_ref-path]/data/CTE-LEAF_LITTER_EL-FAME.txt 2002-11-02 2006-06-09